We’ll start with tartaric acid, which is the stronger of the two and has pKa values of 2.95 and 4.25. First, an acid’s ability to reduce the pH of a solution to values much below that of the pKa value of the acid is limited. Using the pKa values, one can see lactic acid is a stronger acid than acetic acid. The oral dose was well-absorbed. EC number: 212-769-1 The plots of ionization state also show that each of these acids may be present in two or three differen… Identification numbers. It is a conjugate acid of a D-tartrate(1-). The key difference between tartaric acid and citric acid is that the tartaric acid (cream of tartar, C 4 H 6 O 6) is diprotic whereas the citric acid (C 6 H 8 O 7) is triprotic. Assuming that at a particular point in the fermentation process the concentration of potassium hydrogen tartrate is twice that of tartaric acid, calculate the pH of the wine. Tartaric acid (also known as dihydroxybunedioic acid or dicarboxylic acid) is a white crystalline organic acid. pKa of Tartaric acid … During the fermentation of wine, a buffer system consisting of tartaric acid and Potassium hydrogen tartrate is produced in a biochemical reaction. is the ionization equation: HC6H4NO2+ Math. Buffering properties : Citric acid is a good buffering agent for solutions between about pH 2 and pH 8. Calculate the amount of tartaric acid used in the recipe. A chemist needs to mix an 18% acid solution with a 45% acid solution to obtain a 12 liter mixture consisting of 36% acid. Second, the presence of ionized and un-ionized acid states in a solution provides some buffering power. For details on it (including licensing), click here. Acids in Wine Part 3: Illustrating the Effects of Ionization States. It is also one of the main acids found in wine. In malic acid, the difference in pKa values (pKa2 – pKa1) is greater than that difference for tartaric acid, so the blue and yellow lines defining the fractions of the un-ionized and doubly-ionized forms of the acid are spaced further apart. 1 Structures Expand this section. D-tartaric acid: Overview. Median response time is 34 minutes and may be longer for new subjects. We will find in what follows that—under some conditions—our acids may not be acidic. Oral or parenteral doses of monosodium 14C-L(+)-tartrate (400 mg/kg) are rapidly excreted by rats and a proportion completely metabolized to CO2. Lethal Dose/Conc 50% kill: The blue line shows the fraction of the acid present in the un-ionized state (the non-dissociated lactic acid) and the red line shows the fraction of the acid present in the ionized state (the lactate ion). In a previous article (Acids in Wine Part 1: Overview), we attempted to cover a lot of ground relative to the subject of acids in general and the ones likely to be found in our juices and wines in particular. It is a chiral molecule and shows stereoisomerism properties namely, D-tartaric acid, L-tartaric acid, and meso-tartaric acid. On the other hand, if the lactic acid molecule exists in solution as a complete molecule with the loosely-bonded proton in place, that molecule has no effect on the pH of the solution. This self-regulation or buffering activity is restricted to the range of pH values within which there are significant quantities of the acid in each ionization state. And Herrick, I.W. As a result of that greater separation of the two outer curves, the red line defining the singly-ionized state's fraction achieves a greater magnitude than for tartaric acid. Its salt, potassium bitartrate, commonly known as cream of tartar, develops naturally in the process of fermentation. During the fermenation of wine, a buffer system consisting of tartaric acid and potassium hydrogen tartrate is produced by a biochemical reaction. Acidul tartric (acidul 2,3-dihidroxibutandioc) este un acid organic, diprotic, cu formula HOOC-CHOH-CHOH-COOH.Acesta este un dihidroxi-derivat al acidului succinic. *Response times vary by subject and question complexity. Finally, the plots illustrate the fact that you cannot use a weak acid to get a really low pH since, at low pH values, a weak acid is not ionized; thus further additions of the acid results in no additional hydrogen ions in solution. It is also known as 2,3-dihydroxysuccinic acid or Racemic acid. There are 2 dissociation equilibria known as (pKa’s). Recall that the bi-functional descriptor means that each of these acids has two [-COOH] groups which can each lose their terminal hydrogen nuclei to the liquid in which they are dissolved. In solution, each molecule of the lactic acid must exist in one of two states: as a complete molecule with the loosely-bonded hydrogen atom still attached, or as an ion pair consisting of a positively-charged hydrogen nucleus and a negatively-charged lactate ion. We’ll start with tartaric acid, which is the stronger of the two and has pKa values of 2.95 and 4.25. Changes in ionization states of acids and stationary phase can be used to control elution order of organic and inorganic acids. Enjoy! It may also be used as a ligand to synthesize chiral transition metal complexes, which have potential utility in organic asymmetric catalysis. Less soluble in water than L-tartaric acid.pH of 0.1M aq soln: 2.0. This table lists the acid-base dissociation constants of over 600 organic compounds, including many amino acids. May affect kidneys (kidney For example, if 10 grams of tartaric acid is used, divide 10 grams by 4. All data apply to dilute aqueous solutions and are presented in the form of pK a , which is the negative of the logarithm of the acid dissociation constant K a . Calculate the amount of tartaric acid used in the recipe. Type. Other Toxic Effects on Humans: in wines or juices and that that condition leads to buffering of the liquids' pH values—since external changes to a solution’s pH are counteracted by changes in the acids’ ionization states. Tartaric acid is a white crystalline diprotic organic acid. Acute Potential Health Effects: Next we are going to look at the ionization states of malic acid, which has a molecular structure very similar to that of tartaric acid. More... Validated by Experts, Validated by Users, Non-Validated, Removed by Users. This information should not be interpreted without the help of a healthcare provider. We invite interested applicants to inquire by emailing Prof. Zhang. A severity rating for each drug interaction, from minor to major. Dibenzoyl-L-tartaric acid may be used as a chiral resolving agent for the resolution of racemic Troger base. If you adjust the $\mathrm{pH}$ to 6.4, tartaric acid would be roughly 100% deprotonated, while 50% of citric acid still has one proton to give off. For bullet point notes on the acid distinctions, please see the above drawing. (Assume the change is volume isnegligible.) Chemical formula: C 4 H 5 KO 6 Molecular mass: 188.18. The main difference between tartaric acid and citric acid is that the tartaric acid naturally occurs in grapes whereas citric acid naturally occurs in citrus fruits.. Tartaric acid and citric acid are two types of plant acids used as natural food additives. What is Tartaric Acid? Ingestion: Repeated or prolonged ingestion may cause lesions of the mouth, gastric ulcers, gastrointestinal Properties: Anhydr acid, triclinic pinacoidal crystals from abs alc, from water above 73°, or by drying the monohydrate at 100°. Citric acid (2-hydroxypropane-1,2,3-tricarboxylic acid) is a weak organic tricarboxylic acid with three different values of pKa (3.1, 4.7, and 6.4). Based on given acidity constants (pK a values) the pH of organic acids for 1, 10, and 100 mmol/L are calculated.The results are listed in the following tables (valid for standard conditions 25°C, 1 atm):. The therapeutic efficacy of Eravacycline can be decreased when used in combination with L-tartaric acid. Figure 2—Tartaric Acid. In high doses, this agent acts as a muscle toxin by inhibiting the production of malic acid, which could cause paralysis and maybe death. Above pH 3.8, it’s mostly ionized, and below 3.8, it’s mostly undissociated. Its salt, potassium bitartrate, commonly known as cream of tartar, develops naturally in the process of winemaking. Figure 1—Lactic Acid. In contrast to what we saw with tartaric acid, Figure 3 shows that at typical wine or juice pH levels, there would be essentially no doubly-ionized malic acid present. This is “Appendix C: Dissociation Constants and pKa Values for Acids at 25°C”, appendix 3 from the book Principles of General Chemistry (v. 1.0). The plots of ionization state also show that each of these acids may be present in two or three different ionization states (including un-ionized!) Substance identity Substance identity. Tartaric Acid is a carboxylic acid with a chemical formula C 4 H 6 O 6. DL-Tartaric acid. It is also used as an antioxidant. Tartaric acid is commercially available as a white powder and has a very poor water solubility while citric acid is an odorless compound and is available as a solid crystalline compound. There are 2 dissociation equilibria known as (pKa’s). Tartaric acid is a white crystalline organic acid that occurs naturally in many plants, most notably in grapes.Tartaric is an alpha-hydroxy-carboxylic acid, is diprotic and aldaric in acid characteristics, and is a dihydroxyl derivative of succinic acid. Without the concentration of the solution we cannot say the Ph, since Ph is determined for a solution. LorAnn Tartaric Acid Powder (Tart), 3.4 Ounce. Eyes: Causes eye irritation Since Tartaric acid has two H+ ions that can be released in solution, depending on pH. The pKa of tartaric acid is 2.96. Compound Type Concentration (R,R)-Tartaric acid: Solute: 100mM: D2O: Solvent: 100%: sodium phosphate: Buffer: 50mM: sodium azide: Cytocide: 500uM: DSS: Reference: 500uM Acidul tartric poate fi găsit în numeroase plante, și în numeroase fructe, precum strugurii și bananele. Property Value Probability; Human Intestinal Absorption-0.532: Blood Brain Barrier • As an additive in electrochemical deposition technique for the synthesis of bismuth thin films to be used as X-ray absorbers. In the previous article, we attempted to make the following points: We’re going to look first at the ionization states of the simplest type of acid of interest to us—the mono-functional lactic acid whose molecular structure contains a single [-COOH] group with the loosely-held hydrogen atom at the end. At the highest levels of pH in the chart, all the acid is present as the lactate ion so all the acidic protons are separated and the acid would be having its maximum effect on the pH of the solution. From a chemist’s perspective, an acid is a molecule which dissociates or ionizes in solution to yield free hydrogen nuclei. Small Molecule. In this chart the lower pKa value is indicated by the point at which the blue and red lines cross, and the higher pKa value is indicated by the point at which the red and yellow lines cross. In most plants, this organic acid is rare, but it … The therapeutic efficacy of Doxycycline can be decreased when used in combination with L-tartaric acid. It should be apparent that the reason tartaric acid is recommended for efficiently lowering the pH of wines is that is has the lowest pKa value—and the largest pH-lowering effect—of the common fruit acids. Tartaric Acid is a white crystalline dicarboxylic acid found in many plants, particularly tamarinds and grapes. The chart and those shown later were obtained via calculations utilizing some equations which were derived through a process illustrated in The Effect of Malate or Lactate Content on the pH-TA Relationship of Potassium Bitartrate Saturated Alcohol-Water Solutions (Nagel, C.W. Packaging 25, 100 g in poly bottle Contraindications & Blackbox Warnings data. At 25° pKa 1 2.98; pKa 2 4.34. pH of 0.1N soln: 2.2. Tartaric Acid is an organic acid is found in many vegetables and fruits such as bananas, grapes, but also in bananas, citrus, and tamarinds. ChEBI. The culinary arts, photography, tanning, ceramics, mirror making, and medicinal remedies make use of tartaric acid. 2Ta (tartaric acid), ú HTa-(is a Bitartrate ion), and ú Ta2 - (is a Tartrate ion) Tartaric acid has two acid (-COOH) groups, that can lose their terminal hydrogen in solution. Figure 2—Tartaric Acid. At room temperature, citric acid is a white hygroscopic crystalline powder (Figure 4A.31). What is the acid-ionization constant, Ka and pKa for this acid at 25*C? The therapeutic efficacy of Demeclocycline can be decreased when used in combination with L-tartaric acid. [. ... Tartaric acid has two acidic (ionizable) hydrogens. Next we are going to look at the ionization states of more complicated bi-functional acids which are dominant in grapes: tartaric and malic. Originally, it was commonly extracted from tamarinds in areas including Nigeria and Sudan to be used for its health benefits and as a food ingredient. Tartaric acid is not very strong, pKa for natural L(+)-isomer are 2.9 and 4.4 (there are two values since this acid has 2 COOH groups). The D-enantiomer of tartaric acid. organic acids – sorted by formula The excretion of Iofetamine I-123 can be decreased when combined with L-tartaric acid. At even lower values of pH the inefficiency is even greater: at a pH of 2.0, only about 1.4% of the lactic acid present (or added) would be ionized and able to affect the solution pH. Check isomerism to know more about stereoisomerism property of the isomers along with their classifications. Table of Acids with Ka and pKa Values* CLAS Acid HA A-Ka pKa Acid Strength Conjugate Base Strength Hydroiodic HI I-Hydrobromic HBr Br-Perchloric HClO4 ClO4-Hydrochloric HCl Cl-Chloric HClO3 ClO3-Sulfuric (1) H2SO4 HSO4-Nitric HNO3 NO3-Strong acids completely dissociate in aq solution (Ka > 1, pKa … Lactic acid, for example, has a pKa of 3.8. Learn More. However, starting at a pH of about 2 the fraction of the acid present as the lactate ion (the red line) begins to increase as the fraction of the non-dissociated acid begins to decrease. Both … There is additional data available for commercial users including Adverse Effects, Contraindications, and Blackbox Warnings. It is also known as 2,3-dihydroxysuccinic acid or Racemic acid. There are a couple practical effects which may be understood with the aid of a chart like that above. pKa values are 5.21, 4.28 and 2.92 at 25 °C (extrapolated to zero ionic strength). In terms of sensation, the tartaric acid lit up my whole head like a light bulb in bright sunshine yellow light and a high note pitch of energy. It is an enantiomer of a L-tartaric acid. Strong acid taste. Grape is the natural source of this acid. pH of Organic Acids and Salts. Divide the amount of tartaric acid used by 4. The excretion of Amphetamine can be decreased when combined with L-tartaric acid. *Response times vary by subject and question complexity. Contents. Less soluble in water than L-tartaric acid.pH of 0.1M aq soln: 2.0. To substitute citric acid for tartaric acid, use a quarter of the amount of tartaric acid required, in this example 2.5 grams of citric acid. 40, No. Some acids have multiple pKas. A solution’s pH is really just a measure of the concentration of those free hydrogen nuclei in the solution. Outside of the human body, D-Tartaric acid has been detected, but not quantified in, loquats. pKa Definition . Tartaric acid is a white, crystalline organic acid that occurs naturally in many fruits, most notably in grapes, but also in bananas, tamarinds, and citrus. Current: Postdoctoral research position available. The chart shows that acids with different values of pKa … Tartaric Acid is an organic acid is found in many vegetables and fruits such as bananas, grapes, but also in bananas, citrus, and tamarinds. D-Tartaric acid is an extremely weak basic (essentially neutral) compound (based on its pKa). Property Value Probability; Human Intestinal Absorption-0.532: Blood Brain Barrier Tartaric acid can be added to food when a sour taste is desired. While tartaric acid is commonly found in foods such as grapes and apricots, it wasn’t until later that this was discovered. General description (+)-O,O′-Diacetyl-L-tartaric anhydride is an HPLC derivatization reagent for UV/Vis detection.It is mainly employed as a reagent for the chiral derivatization of amino alcohols. Calculate the[C 4 H 4 O 6 2-] inthe resulting solution. Tartaric acid is, from a winemaking perspective, the most important in wine due to the prominent role it plays in maintaining the chemical stability of the wine and its color and finally in influencing the taste of the finished wine. What is the acid-ionization constant, Ka and pKa for this acid at 25*C? We see once again that at the lowest levels of pH, tartaric acid exists in solution in the un-ionized state, but at pH levels between the two pKa values, the singly-ionized state is the most populated, and then at pH levels much above the higher pKa value most of the acid is present in the doubly-ionized state. It comes from a naturalcrystalline acid deposited on the inside of the wine barrels at the cellars. Divide the amount of tartaric acid used by 4. Tartaric acid is a stonger acid than malic acid and hence the choice for addition to reduce grape juice and wine pH values. pKa = -log 10 K a The lower the pK a value, the stronger the acid.For example, the pKa of acetic acid is 4.8, while the pKa of lactic acid is 3.8. $6.99 $ 6. Strong organic acid. Seven carboxylic acids are separated based on their polarity and pKa values. It is used to generate carbon dioxide. Inhalation: Causes respiratory tract irritation Chronic Potential Health Effects: Tartaric acid is a white crystalline organic acid that occurs naturally in many plants, most notably in grapes.Tartaric is an alpha-hydroxy-carboxylic acid, is diprotic and aldaric in acid characteristics, and is a dihydroxyl derivative of succinic acid. Tartaric Acid is a carboxylic acid with a chemical formula C 4 H 6 O 6. 06/15/2020: The Zhang Lab has officially re-opened in accordance with state and university sanctioned safety practices after a three month shutdown due to COVID-19. From the chart we see that at the pKa value for lactic acid, only 50% of the acid (or of further additions of acid) is ionized and thus able to lower the pH further. It can exist either in an anhydrous (water-free) form or as a monohydrate. D-tartaric acid is the D-enantiomer of tartaric acid. At a pH of just under 4 in the chart, the two lines cross and at that point each state is present at a fraction of 0.5 or 50%. damage), blood, and behavior (convulsions, somnolence), and respiration. [a] D20 +12.0° (c = 20 in H 2 O). Drug created on November 30, 2015 12:10 / Updated on June 12, 2020 10:52. Extended description of the mechanism of action and particular properties of each drug interaction. Note in the chart for tartaric acid (Figure 2) that three curves are required to define the three possible ionization states for the tartaric acid. The concentration of the positively-charged hydrogen nuclei (aka protons) in the solution is what we measure when we measure pH, and the higher the concentration, the lower the pH. The ‘Substance identity’ section is calculated from substance identification information from all ECHA databases. To substitute citric acid for tartaric acid, use a quarter of the amount of tartaric acid required, in this example 2.5 grams of citric acid. Tartaric acid is a diprotic acid and requires two molecules of NaOH to react with one molecule of tartaric acid (see equation). What is tartaric acid used for? Median response time is 34 minutes and may be longer for new subjects. Tartaric acid has been in use since Greek and Roman times, and for good reason. InChI=1S/C4H6O6/c5-1(3(7)8)2(6)4(9)10/h1-2,5-6H,(H,7,8)(H,9,10)/t1-,2-/m1/s1. There are likely many potential references from which the required equations may be obtained, but the advantage to me of this particular reference is that it presents a derivation of the required equations so that I could understand their origin. For details on it (including licensing), click here. Tartaric acid is a dihydroxy and dicarboxylic acid as it has two OH and two COOH groups. Salts of tartaric acid are known as tartarates. Since malic and tartaric acid both have two protons which may be lost to the solution, there are two pKa values for each acid. The blue line represents the fraction of the acid that is un-ionized, the red line represents the singly-ionized acid, and the yellow line represents the doubly-ionized acid. AJEV, Vol. DrugBank's structured data is collected, curated and updated daily by our team of pharmacists, pharmacologists and medical doctors. Chemists use the concept of pKa as an indicator of an acid’s strength, and each acid’s pKa value(s) provides some indication of the degree to which each acid is ionized. Ever since, the acid has heavily em… A chemist needs to mix an 18% acid solution with a 45% acid solution to obtain a 12 liter mixture consisting of 36% acid. Calculate the value of the firstdissociation constant, K 1, for tartaric acid if thevalue of the second dissociation constant, K 2, is4.60x10-5.. C.) To a 0,015 molar solution of tartaric acid, a strong acidis added until the pH is 0.5. The blue line represents the fraction of the acid that is un-ionized, the red line represents the singly-ionized acid, and the yellow line represents the doubly-ionized acid. Cream of Tartaris a fine powder also known as Potassium Bitartratemanufactured from natural raw materials. pKa 1 2.96; pKa 2 4.24. 99 ($3.50/Ounce) Save more with Subscribe & Save. If you believe you are experiencing an interaction, contact a healthcare provider immediately. Tartaric acid is a white crystalline diprotic organic acid. Its salt, the potassium hydrogen tartrate, is called the cream of tartar. It is used to generate carbon dioxide. LDL [Rat - Route: oral; Dose: 7500 mg/kg In this article we will dig a little more deeply into one of the concepts introduced in the earlier article—that of pKa—and hopefully thereby illustrate how that important property can give us some insight into what is going on with the acids in our wines. What is tartaric acid used for? The compound occurs naturally in many plants, particularly in grapes, bananas, and tamarinds. It is also used as an antioxidant. Go to Acids in Wine Part 3: Illustrating the Effects of Ionization States. pKa at 20°C provides numerical substance information on the dissociation constant in pKa at a temperature of 20°C. As reported in the earlier Home Wine Press article, malic acid is a bit weaker than tartaric, and that means that the pKa values for malic (3.4 and 5.2) are a bit higher than those for tartaric. nausea, vomiting, muscle aches, pains, and weakness. Contact us to learn more about these and other features. Heat of combustion: -275.1 kcal/mol. Refreshing when in dil aq soln. 2. The excretion of Lisdexamfetamine can be decreased when combined with L-tartaric acid. (Assume the change is volume isnegligible.) Tartaric acid is used to generate carbon dioxide through interaction with sodium bicarbonate following oral administration. The ‘Substance identity’ section is calculated from substance identification information from all ECHA databases. Lowest Published Lethal Dose: The therapeutic efficacy of Lymecycline can be decreased when used in combination with L-tartaric acid. pK a is the negative base-10 logarithm of the acid dissociation constant (K a) of a solution. What is Tartaric Acid? Properties: Anhydr acid, triclinic pinacoidal crystals from abs alc, from water above 73°, or by drying the monohydrate at 100°. 2Ta (tartaric acid), ú HTa-(is a Bitartrate ion), and ú Ta2 - (is a Tartrate ion) Tartaric acid has two acid (-COOH) groups, that can lose their terminal hydrogen in solution. Specific heat: 0.288 cal/g/°C at 21 to 51°; 0.296 at 0 to 99.6°. Tartaric acid (2,3-dihydroxybutanedioic acid) is a naturally occurring dicarboxylic acid containing two stereocenters. Salts of tartaric acid are known as tartarates. The excretion of Diethylpropion can be decreased when combined with L-tartaric acid. Ingestion: Causes gastrointestinal tract irritation with nausea, vomiting and diarrhea. Tartaric acid is a white, crystalline organic acid that occurs naturally in many fruits, most notably in grapes, but also in bananas, tamarinds, and citrus. Odor of burnt sugar when heated to mp. 2, 1989). This could make D-tartaric acid a potential biomarker for … Pure Tartaric Acid ⊘ Non-GMO Gluten-Free ☮ Vegan OU Kosher Certified - 50g/2oz. Tartaric acid can be added to food when a sour taste is desired. Tartaric acid is used in the manufacture of a range of foods and drinks, and also has industrial applications such as silvering mirrors or tanning leather. Its salt, potassium bitartrate, commonly known as cream of tartar, develops naturally in the process of winemaking. mp 206°. Figure 3—Malic Acid. This plot for malic acid (Figure 3) is quite similar to the earlier one for tartaric acid except that here the curves are shifted somewhat to the right—as should be expected, given the higher pKa values which are indicated once again by the crossings of the yellow and red lines and of the red and blue lines. In the chart for lactic acid we see that at the lowest levels of pH all of the acid is present in the un-ionized state, so that under those conditions, the acid would have no effect on the pH of a solution which contains the acid. mp 206°. Carbohydrates and carbohydrate conjugates, Short-chain hydroxy acids and derivatives, GC-MS Spectrum - GC-EI-TOF (Pegasus III TOF-MS system, Leco; GC 6890, Agilent Technologies), splash10-0002-0930000000-19235937e21066a9c484, splash10-000f-0961000000-559c31b016f4fb3700d2, splash10-0007-0961000000-1b17d674621eb9d88c3c, MS/MS Spectrum - Quattro_QQQ 10V, Negative, splash10-000b-7900000000-d247ec12b77f427e9a76, MS/MS Spectrum - Quattro_QQQ 25V, Negative, splash10-05fu-9000000000-4bf01ad116d24a453817, MS/MS Spectrum - Quattro_QQQ 40V, Negative, splash10-0006-9000000000-4da65da10f3a34f77688, LC-MS/MS Spectrum - LC-ESI-QQ (API3000, Applied Biosystems) 10V, Negative, splash10-0002-0900000000-ecb902731b9f6eb3764d, LC-MS/MS Spectrum - LC-ESI-QQ (API3000, Applied Biosystems) 20V, Negative, splash10-0072-9700000000-1e8e6f410c1d71af856e, LC-MS/MS Spectrum - LC-ESI-QQ (API3000, Applied Biosystems) 30V, Negative, splash10-00di-9000000000-f13ad2560d3e6818db9c, LC-MS/MS Spectrum - LC-ESI-QQ (API3000, Applied Biosystems) 40V, Negative, splash10-05fu-9000000000-f46a01eb933ba6fbceac, LC-MS/MS Spectrum - LC-ESI-QQ (API3000, Applied Biosystems) 50V, Negative, splash10-0006-9000000000-08dec35ca6a80add253a, Predicted MS/MS Spectrum - 10V, Positive (Annotated), Predicted MS/MS Spectrum - 20V, Positive (Annotated), Predicted MS/MS Spectrum - 40V, Positive (Annotated), Predicted MS/MS Spectrum - 10V, Negative (Annotated), Predicted MS/MS Spectrum - 20V, Negative (Annotated), Predicted MS/MS Spectrum - 40V, Negative (Annotated), Galactosylgalactosylxylosylprotein 3-beta-glucuronosyltransferase 1, Hypoxia-inducible factor 1-alpha inhibitor.